1. Female Patient : Remove any excess mucus from the potentially infected site with the first swab, and then discard it.

2. Rub the second swab vigorously over the infected end urethral lining and end cervical cells in the canal wall.

3. As Chlamydia is intracellular organisms, firm contact must be made with the canal wall for proper specimen collection. The rubbing action dislodges the endothelial cells and allows the swab to absorb the bacteria. Improper collection will result in poor visual readings and may cause invalid results.

2. Carefully remove the swab avoiding contact with any external surfaces. Urine specimen: Female patients should not cleanse the labial area prior to providing the specimen. First-void urine (patient should not have urinated for 1 hour prior to specimen collection) or endocervical/urethral swab specimen.

Urine: Collect 15ml urine specimen; centrifuge the urine specimen at high speed (1,500-3,000 rpms) for 15 minutes. Discard the supernate. Use the precipitate for detection. Treat the urine sediment according to Direction for Use, if the test is to be conducted immediately.